Qualifications of Founder

Publications

Tafur AJ, McBane RD, Ammash N, Asirvatham SJ, Miller RD, Janczak D, Slusser JP, Grill DE, Whelan SL, Wysokinski WE. Impact of Atrial Fibrillation and Sinus Rhythm Restoration on Reticulated Platelets.

Mayo Clin Proc. 2015 Oct 30. pii: S0025-6196(15)00765-X. doi: 10.1016/j.mayocp.2015.09.013.

Waldemar E. Wysokinski, Alfonso Tafur, Thomas G. McLeod, Naser Ammash, Win-Kuang Shen, Diane D. Grill, Jessica Sonbert, SL Whelan, Jozef Mruk, and Robert D. McBane, II .

Impact of Atrial Fibrillation on Platelet-Predominate Gene Expression

J Am Coll Cardiol, 2011; 57:1518, doi:10.1016/S0735-1097(11)61518-0

Alfonso J Tafur; Robert McBane, II; Mruk J; Nasser Ammash; Shen Win-Kuang; Whyte G Owen;

SL Whelan; Waldemar Wysokinski.

Abstract 16294: Atrial Fibrillation is Associated with Higher Percentage of Young Platelets.

American Heart Association, 2010; 122: A16294

Christopher G A McGregor, Henry D Tazelaar, Vinay P Rao, Davide Ricci, Naoto Miyagi, Mozammel Gazi, Shelly Whelan, Sarah Edgerton, and Guerard W Byrne

Gene-knockout (GT-KO) heterotopic cardiac xenotransplantation: are GT-KO pigs essential for successful clinical xenotransplantation?

Xenotransplantation 14(2):181 (2007) DOI: 10.1111/j.1399-3089.2007.00386_6.x


Woollard JR, Punyashtiti R, Richardson S, Masyuk TV, Whelan S, Huang BQ, Lager DJ, vanDeursen J, Torres VE, Gattone VH, LaRusso NF, Harris PC, Ward CJ.
A mouse model of autosomal recessive polycystic kidney disease with biliary duct and proximal tubule dilatation. Kidney Int. 2007 Aug;72(3):328-36. Epub 2007 May 23.

Smith UM, Consugar M, Tee LJ, McKee BM, Maina EN, Whelan S, Morgan NV, Goranson E, Gissen P, Lilliquist S, Aligianis IA, Ward CJ, Pasha S, Punyashthiti R, Malik Sharif S, Batman PA, Bennett CP, Woods CG, McKeown C, Bucourt M, Miller CA, Cox P, Algazali L, Trembath RC, Torres VE, Attie-Bitach T, Kelly DA, Maher ER, Gattone VH 2nd, Harris PC, Johnson CA.

The transmembrane protein meckelin (MKS3) is mutated in Meckel-Gruber syndrome and the wpk rat. Nat Genet. 2006 Feb;38(2):191-6. Epub 2006 Jan 15.


Gattone VH 2nd, Tourkow BA, Trambaugh CM, Yu AC, Whelan S, Phillips CL, Harris PC, Peterson RG.
Development of multiorgan pathology in the wpk rat model of polycystic kidney disease.
Anat Rec A Discov Mol Cell Evol Biol. 2004 Apr;277(2):384-95.

Ward CJ, Yuan D, Masyuk TV, Wang X, Punyashthiti R, Whelan S, Bacallao R, Torra R, LaRusso NF, Torres VE, Harris PC.

Cellular and subcellular localization of the ARPKD protein; fibrocystin is expressed on primary cilia.

Hum Mol Genet. 2003 Oct 15;12(20):2703-10. Epub 2003 Aug 12. PMID: 12925574 [PubMed ]

Poduslo JF, Whelan SL, Curran GL, Wengenack TM.

Therapeutic benefit of polyamine-modified catalase as a scavenger of hydrogen peroxide and nitric oxide in familial amyotrophic lateral sclerosis transgenics.

Ann Neurol. 2000 Dec;48(6):943-7. PMID: 11117554 [PubMed - indexed for MEDLINE]

Wengenack TM, Whelan S, Curran GL, Duff KE, Poduslo JF.

Quantitative histological analysis of amyloid deposition in Alzheimer's double transgenic mouse brain.

Neuroscience. 2000;101(4):939-44. PMID: 11113343 [PubMed - indexed for MEDLINE]


Work Experience of Founder

Sr. Research Technologist, Mayo Clinic, Rochester, MN Jan 2006 – Mar 2010

  • Development and modification of methodology for the characterization of platelets from human blood and the analysis of these platelets using flow cytometry

  • Train and supervise staff and students in newly devised techniques

  • Animal genomic DNA and RNA analysis

  • Surgical procedures with animals including perfusion, collection of organs and tissues, dissection of most organ systems for expression analysis, and implanting medical devices

  • Analysis and publication of data using statistical software as well as multiple computer systems and programs for data presentation, record keeping, and primer design


Research Technologist, Mayo Clinic, Rochester, MN May 2001 – Jan 2006

  • Design and administration of breeding strategies and phenotypic analysis of affected animals in novel mutational analysis, PCR, Southern, and Northern analysis

  • Qualitative and quantitative trait analyses of linkage mapping for cloning the novel gene mutation Wpk using UNIX / Artemis

  • Multifactorial experimental design and SAS analysis of data for reporting in scientific journals

  • Sanger and NGS methods to find mutation and expression variables between background models. Variability mapping for experimental candidates for novel CHIP assay


Research Technologist, Mayo Clinic, Rochester, MN July 1998-May 2001

  • Development and support of comprehensive customized databases (Filemaker Pro)

  • High volume, time critical processing of animal DNA by PCR

  • Genotype determination under time constraints

  • Independent optimization and troubleshooting of complex PCR

Education of Founder

Winona State University, Winona, MN Jan 2018 – March 2020

Professional Science Masters, Bioinformatics and CLS (In Progress)

Rochester Community and Technical College, Rochester, MN Jan 2015 – Dec 2016

Certificate, Healthcare Informatics (High Honors-GPA: 3.98)

Rochester Community and Technical College, Rochester, MN Jan 2013 – May 2014

Associate of Science, Computer Science (High Honors-GPA: 3.75)

University of Minnesota, Minneapolis, MN Sep 1994 – Aug 1997

Masters of Science, Plant Breeding (GPA 3.27)

University of Minnesota, Minneapolis, MN Sep 1992 – Aug 1994

Bachelors of Science, Biology, Genetics and Cell Biology (Honors-GPA: 3.43)

PHI THETA KAPPA - Inducted Fall 2013